Part:BBa_K4628018

subPromoter CP

Usage and Biology

The 3’ border of the core CP sgRNA promoter was mapped between -26 and -69 and the 5’ border was +12, whereas the fully active CP sgRNA promoter was mapped between -157 and +54. Computer-predicted folding of fully active CP-sgRNA promoters reveals a long stem ring structure. Deletion analysis showed that the upper part of the stem ring upstream of the transcription initiation site was essential for transcription, while the lower part of the stem had an enhancement effect.

This Part is derived from the subgenomic coat protein promoter in the TMV genome, which mediates the transcription of CP. Because part of the coding sequence of capsid protein constitutes the sequence of subPromoter CP, we mutant the start codon in the subpromoter(5,712–5,714,from ATG to AGA) in case of expressing a nonsense peptide

By mutating the start codon of CP protein coding sequence within promoter sequence, we successfully used the modified promoter to express GFP that can emit light correctly(Fig.1).

Figure 1 subPromoter:GFP in the tobacco leaf

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]