Part:BBa_K4628017

TLS(Ara.Met.ΔΔt.muti)

TLS(Ara.Met.ΔΔt.muti)

Introduction

In RNA imaging systems for MS2/MCP^[1], sequence-specific RNAs that bind to MS2 viral capsid proteins are often used to enhance binding to proteins by increasing the copy number, and such RNAs are characterized by the ability to maintain a stable secondary structure after multiple repeats of the stem-loop. pCR4-24xMS2SL-stable is a commonly used plasmid for the expression of MS2-specific binding RNA, which produces an RNA(SL24) containing 24 stem-loop repeat structures^[2]. Expecting to enhance mRNA binding to RNA motility-associated proteins by increasing the copy number of the TLS sequence, we replaced some of the repeats of SL24 with the RNA sequence of TLS (Ara.Mer.ΔΔt).

Fig 1. Headphone results for TLS(Ara.Met.ΔΔt.muti) RNA.

Reference

[1] Tyagi S. Imaging intracellular RNA distribution and dynamics in living cells. Nat Methods. 2009 May;6(5):331-8.

[2] Bertrand E, Chartrand P, Schaefer M, Shenoy SM, Singer RH, Long RM. Localization of ASH1 mRNA particles in living yeast. Mol Cell. 1998 Oct;2(4):437-45.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]