Part:BBa_K4628010

eGFP

Enhanced Green Fluorescent Protein (eGFP) is a genetically engineered variant of the Green Fluorescent Protein (GFP), derived from Aequorea victoria. eGFP emits bright green fluorescence when excited by ultraviolet or blue light, allowing for imaging and tracking purposes.

Usage and Biology

The major excitation peak of eGFP is 488 nm, and the peak emission is 509 nm. EGFP has an extinction coefficient (denoted ε) of 55,000 M⁻¹cm⁻¹. The fluorescence quantum yield (QY) of EGFP is 0.60. The relative brightness, expressed as ε•QY, is 33,000 M⁻¹cm⁻¹. eGFP can be widely used as a reporter in a variety of organisms, such as bacteria, plants, animal cells.

Fig 1. Emission and excitation spectra of eGFP from FPbase( https://www.fpbase.org/protein/egfp/)

Expression of eGFP:

We expressed eGFP protein in plant cells. And we observed the expression of eGFP in plant cells by fluorescence microscopy, and the expression of eGFP in plant tissues by LUYOR-3415RG portable fluorescent protein excitation light source as well as filters.

Fig 2. Expression of eGFP in Nicotiana benthamiana. a. Under fluorescence microscope observation, the expression of eGFP in plant cells; b. Observation of eGFP expression in plant tissues using a filter under LUYOR-3415RG portable fluorescence protein extraction light source excitation.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]