Coding

Part:BBa_K4628008:Design

Designed by: Qinyao Yan   Group: iGEM23_ZJU-China   (2023-10-11)


Pikm-1*(Enhancer)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1496
    Illegal SpeI site found at 2070
    Illegal SpeI site found at 2076
    Illegal PstI site found at 2289
    Illegal PstI site found at 2505
    Illegal PstI site found at 2520
    Illegal PstI site found at 2589
    Illegal PstI site found at 3033
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3619
    Illegal SpeI site found at 2070
    Illegal SpeI site found at 2076
    Illegal PstI site found at 2289
    Illegal PstI site found at 2505
    Illegal PstI site found at 2520
    Illegal PstI site found at 2589
    Illegal PstI site found at 3033
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2725
    Illegal BamHI site found at 2350
    Illegal XhoI site found at 3183
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1496
    Illegal SpeI site found at 2070
    Illegal SpeI site found at 2076
    Illegal PstI site found at 2289
    Illegal PstI site found at 2505
    Illegal PstI site found at 2520
    Illegal PstI site found at 2589
    Illegal PstI site found at 3033
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1496
    Illegal SpeI site found at 2070
    Illegal SpeI site found at 2076
    Illegal PstI site found at 2289
    Illegal PstI site found at 2505
    Illegal PstI site found at 2520
    Illegal PstI site found at 2589
    Illegal PstI site found at 3033
    Illegal NgoMIV site found at 721
    Illegal NgoMIV site found at 736
    Illegal NgoMIV site found at 2104
    Illegal NgoMIV site found at 3201
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2334
    Illegal SapI site found at 1272
    Illegal SapI.rc site found at 531
    Illegal SapI.rc site found at 3242


Design Notes

The precise positioning of the ID sequence determines the ability to alter the molecular recognition spectrum without compromising its original immune activation function. Some data form NCBI helped a lot.


Source

Laboratory artificial synthesis.

References