Coding

Part:BBa_K4628007:Design

Designed by: Qinyao Yan   Group: iGEM23_ZJU-China   (2023-10-11)


Pikm-1(TS)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1367
    Illegal SpeI site found at 1941
    Illegal SpeI site found at 1947
    Illegal PstI site found at 2160
    Illegal PstI site found at 2376
    Illegal PstI site found at 2391
    Illegal PstI site found at 2460
    Illegal PstI site found at 2904
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3490
    Illegal SpeI site found at 1941
    Illegal SpeI site found at 1947
    Illegal PstI site found at 2160
    Illegal PstI site found at 2376
    Illegal PstI site found at 2391
    Illegal PstI site found at 2460
    Illegal PstI site found at 2904
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2596
    Illegal BamHI site found at 2221
    Illegal XhoI site found at 3054
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1367
    Illegal SpeI site found at 1941
    Illegal SpeI site found at 1947
    Illegal PstI site found at 2160
    Illegal PstI site found at 2376
    Illegal PstI site found at 2391
    Illegal PstI site found at 2460
    Illegal PstI site found at 2904
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1367
    Illegal SpeI site found at 1941
    Illegal SpeI site found at 1947
    Illegal PstI site found at 2160
    Illegal PstI site found at 2376
    Illegal PstI site found at 2391
    Illegal PstI site found at 2460
    Illegal PstI site found at 2904
    Illegal NgoMIV site found at 1975
    Illegal NgoMIV site found at 3072
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 788
    Illegal BsaI site found at 2205
    Illegal BsaI.rc site found at 742
    Illegal SapI site found at 1143
    Illegal SapI.rc site found at 531
    Illegal SapI.rc site found at 3113


Design Notes

Used for further modification of its resistance spectrum.


Source

Downloaded from NCBI database https://www.ncbi.nlm.nih.gov/gene/109787202


References