Part:BBa_K4628007

Pikm-1(TS)

A kind of plant NLR receptor protein that can perform specific recognition and immune activation function.

Overview of Pi-km

Pi-km is a major resistance allele on the rice blast resistance site: Pik, consisting of two closely linked NB-LRR genes with independent functions (Pikm1-TS and Pikm2-TS). Pi-km mediated rice blast resistance requires the joint action of Pikm1-TS and Pikm2-TS. When only one of Pikm1-TS or Pikm2-TS is present, rice exhibits susceptibility.

Introduction to Pikm-1

Pikm-1 is the component responsible for serving as the primary immune receptor, consisting of the N-terminal CC domain, the central NB-ARC domain, and the C-terminal LRR domain. Generally, we believe that the N-terminal domain is involved in immune recognition and activation, while the central domain is the active switch controlling the entire NLR protein through ADP/ATP exchange. At last, the C-terminal domain achieves fine regulation of protein activity through flexible folding.

Introduction to ID sequence

Meanwhile, research has found that Pikm-1 molecule has a special protein domain called the ID sequence. This sequence can directly bind to the effector secreted by Pyricularia oryza, thereby achieving specific immunity against rice blast. And the existence of ID sequence also provides a theoretical basis for our resistance engineering modification of NLR molecules.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1367
  Illegal SpeI site found at 1941
  Illegal SpeI site found at 1947
  Illegal PstI site found at 2160
  Illegal PstI site found at 2376
  Illegal PstI site found at 2391
  Illegal PstI site found at 2460
  Illegal PstI site found at 2904
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 3490
  Illegal SpeI site found at 1941
  Illegal SpeI site found at 1947
  Illegal PstI site found at 2160
  Illegal PstI site found at 2376
  Illegal PstI site found at 2391
  Illegal PstI site found at 2460
  Illegal PstI site found at 2904
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2596
  Illegal BamHI site found at 2221
  Illegal XhoI site found at 3054
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1367
  Illegal SpeI site found at 1941
  Illegal SpeI site found at 1947
  Illegal PstI site found at 2160
  Illegal PstI site found at 2376
  Illegal PstI site found at 2391
  Illegal PstI site found at 2460
  Illegal PstI site found at 2904
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1367
  Illegal SpeI site found at 1941
  Illegal SpeI site found at 1947
  Illegal PstI site found at 2160
  Illegal PstI site found at 2376
  Illegal PstI site found at 2391
  Illegal PstI site found at 2460
  Illegal PstI site found at 2904
  Illegal NgoMIV site found at 1975
  Illegal NgoMIV site found at 3072
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 788
  Illegal BsaI site found at 2205
  Illegal BsaI.rc site found at 742
  Illegal SapI site found at 1143
  Illegal SapI.rc site found at 531
  Illegal SapI.rc site found at 3113