Coding

Part:BBa_K4628006

Designed by: Qinyao Yan   Group: iGEM23_ZJU-China   (2023-10-11)


LaM4

A nanoantibody derived from alpaca that specifically recognizes mCherry.

Usage and Biology

Nano antibody is a naturally absent light chain antibody present in the peripheral blood of alpacas, which only contains one heavy chain variable region (VHH) and two conventional CH2 and CH3 regions. Unlike artificially modified single chain antibody fragments (ScFv), they are not prone to adhesion or even aggregation into blocks.

LaM4 is one of the nanobodies which can recognize mCherry.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 385
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
biologySynthesis
chassisNicotiana benthamiana