Composite

Part:BBa_K4624632:Design

Designed by: Theofilos Terzopoulos   Group: iGEM23_Thessaly   (2023-10-02)


AraC/ParaBAD-NSP4-laccase-syfp2-rrnB T1/T7TE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NotI site found at 2807
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1995
    Illegal BglII site found at 2427
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 2027
    Illegal XhoI site found at 2660
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1273
    Illegal NgoMIV site found at 2513
    Illegal NgoMIV site found at 2666
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1394
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1459


Design Notes

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Source

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References