DNA
Part:BBa_K4621081:Design
Designed by: Ruikang Chen Group: iGEM23_SCUT-China (2023-10-05)
CRISPRi-488
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 30
Illegal SpeI site found at 4549
Illegal PstI site found at 18
Illegal PstI site found at 2443
Illegal PstI site found at 2677
Illegal PstI site found at 3889 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4526
Illegal SpeI site found at 4549
Illegal PstI site found at 18
Illegal PstI site found at 2443
Illegal PstI site found at 2677
Illegal PstI site found at 3889 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 482
Illegal BglII site found at 1277
Illegal BglII site found at 4046
Illegal BamHI site found at 222
Illegal BamHI site found at 1571
Illegal BamHI site found at 4514
Illegal BamHI site found at 4719
Illegal XhoI site found at 895
Illegal XhoI site found at 3241 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 30
Illegal SpeI site found at 4549
Illegal PstI site found at 18
Illegal PstI site found at 2443
Illegal PstI site found at 2677
Illegal PstI site found at 3889 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 30
Illegal SpeI site found at 4549
Illegal PstI site found at 18
Illegal PstI site found at 2443
Illegal PstI site found at 2677
Illegal PstI site found at 3889
Illegal NgoMIV site found at 1839
Illegal NgoMIV site found at 2210
Illegal NgoMIV site found at 2413 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3421
Illegal BsaI.rc site found at 4300
Illegal SapI site found at 813
Illegal SapI site found at 2103
Illegal SapI.rc site found at 3300
Design Notes
The effectiveness and GC content.
Source
This part is derived from PZ-ProP-Ect-i8 plasmid.