DNA

Part:BBa_K4621073:Design

Designed by: Ruikang Chen   Group: iGEM23_SCUT-China   (2023-10-05)


Spacer of 441-sgRNA to target gabT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The specificity, effectiveness, GC content, off-targeting, and sgRNA self-complementation of the sgRNA will be taken into account when designing the part.


Source

This part is derived from gabT of the SCUT-3 genome.

References