Coding

Part:BBa_K4621070

Designed by: Ruikang Chen   Group: iGEM23_SCUT-China   (2023-10-04)


dCas9

This dCas9 gene is derived from a novel dCas9-based CRISPRi system in S. coelicolor.[1] In our study, we used it and designed sgRNA to suppress gene expression to achieve higher yields.

Reference

[1] Zhao Y, Li L, Zheng G, Jiang W, Deng Z, Wang Z, Lu Y. CRISPR/dCas9-Mediated Multiplex Gene Repression in Streptomyces. Biotechnol J. 2018 Sep;13(9):e1800121. doi: 10.1002/biot.201800121. Epub 2018 Jul 4. PMID: 29862648.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2212
    Illegal PstI site found at 2446
    Illegal PstI site found at 3658
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2212
    Illegal PstI site found at 2446
    Illegal PstI site found at 3658
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 251
    Illegal BglII site found at 1046
    Illegal BglII site found at 3815
    Illegal BamHI site found at 1340
    Illegal XhoI site found at 664
    Illegal XhoI site found at 3010
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2212
    Illegal PstI site found at 2446
    Illegal PstI site found at 3658
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2212
    Illegal PstI site found at 2446
    Illegal PstI site found at 3658
    Illegal NgoMIV site found at 1608
    Illegal NgoMIV site found at 1979
    Illegal NgoMIV site found at 2182
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3190
    Illegal BsaI.rc site found at 4069
    Illegal SapI site found at 582
    Illegal SapI site found at 1872
    Illegal SapI.rc site found at 3069


[edit]
Categories
Parameters
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