DNA

Part:BBa_K4621029:Design

Designed by: Ruikang Chen   Group: iGEM23_SCUT-China   (2023-10-01)


Spacer of 62-sgRNA to target GQS52_08040


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The specificity, effectiveness, GC content, off-targeting, and sgRNA self-complementation of the sgRNA will be taken into account when designing the part.



Source

This part is derived from GQS52_08040 of the SCUT-3 genome.


References

[1] Larson, M., Gilbert, L., Wang, X. et al. CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Nat Protoc 8, 2180–2196 (2013). https://doi.org/10.1038/nprot.2013.132