Coding

Part:BBa_K4619013:Design

Designed by: Binwen Liu   Group: iGEM23_UCAS-China   (2023-10-12)


Hbd


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 124
    Illegal PstI site found at 1033
    Illegal PstI site found at 1447
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 550
    Illegal PstI site found at 124
    Illegal PstI site found at 1033
    Illegal PstI site found at 1447
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 875
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 124
    Illegal PstI site found at 1033
    Illegal PstI site found at 1447
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 124
    Illegal PstI site found at 1033
    Illegal PstI site found at 1447
    Illegal AgeI site found at 568
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization in <i/>E. coli


Source

https://www.ncbi.nlm.nih.gov/gene/83679382

===References===