Coding

Part:BBa_K4616231

Designed by: Zexu Wang   Group: iGEM23_BIT-China   (2023-10-03)


gal4 is the gene of the GAL4 DBD protein

Usage

gal4 expresses the GAL4 DNA Binding Domain (DBD) protein, which functions as a transcriptional positive regulator of the downstream Pgal1-s promoter. This activation leads to the expression of genes downstream of the Pgal1-s promoter, ultimately increasing the metabolic flux of the required enzymes.

Biology

The gene serves a positive regulator of the expression of galactose-inducing genes, such as gal1, gal2, gal7, gal10, and mel1. These genes encode enzymes responsible for the conversion of galactose to glucose. The protein contains a fungal Zn(2)-Cys(6) binuclear cluster domain.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 706
    Illegal PstI site found at 1349
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1349
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 218
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 706
    Illegal PstI site found at 1349
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 706
    Illegal PstI site found at 1349
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 137
    Illegal BsaI.rc site found at 1813
    Illegal SapI site found at 502


[edit]
Categories
Parameters
None