Regulatory

Part:BBa_K4616168

Designed by: Cheng Zou   Group: iGEM23_BIT-China   (2023-10-03)


Pgal1-s is a common strongly inducible promoter

Usage:

Pgal1-s is highly inducible in the presence of galactose and the absence of glucose, which will turn on the expression of downstream genes.

Biology:

Pgal1-s activates the expression of downstream genes, including those related to sclareol synthesis under blue light control. Additionally, Pgal1-s functions as a reverse promoter in a route associated with santalol synthesis. It yields a complementary structure with PTEF1-transcribed mRNA under blue light irradiation, thereby preventing santalol synthesis.

igem23-part-pgal1-s.png

Figure 1. PCR result. M-marker.1-Pgal1-s(part K4616168,719 bp).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 8
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 394
    Illegal AgeI site found at 605
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None