DNA

Part:BBa_K4615007:Design

Designed by: Xinyi (Marry) Xuan   Group: iGEM23_Toronto   (2023-10-12)


FrdA Knockout Template Donor DNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DNA that has 500 bp homologous to both upstream and downstream of frdA gene region in T-B18 genome.

Annealing Temperature: 53C

GC: 40%


Source

1000 bp of synthesized double strand DNA that has 500 bp homologous to both upstream and downstream of frdA gene region in T-B18 genome.

References