Composite

Part:BBa_K4614108

Designed by: Qianhui Sun   Group: iGEM23_CAU-China   (2023-10-06)


T7 Promoter-RBS-LuxI-B0015 Ter

LuxI is the synthase gene for autoinducers, which, when expressed, can produce signal molecules known as N-acyl homoserine lactones (AHL or HSL). Introducing this pathway into E. coli can be used to produce N-acyl homoserine lactones (AHL or HSL). AHL signal molecules can freely diffuse across the cell membrane, and their concentration increases with the cell density.When the concentration of signal molecules reaches a threshold, they bind to LuxR to form a complex, which activates the transcription of the luciferase operon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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