Composite

Part:BBa_K4613891

Designed by: Qinfei Zhou   Group: iGEM23_NAU-CHINA   (2023-10-10)


YF1-PAL-GFP

This composite part contains BBa_K4613429, BBa_K2656009, BBa_4613168, BBa_B0053, BBa_J64997, BBa_K592016, BBa_B0053, BBa_K2277233, and BBa_E0040, which was used as a control to minimize the effect of variables and PAL.

Reference

  1. Ranzani A T, Wehrmann M, Kaiser J, et al. Light-dependent control of bacterial expression at the mRNA level[J]. ACS Synthetic Biology, 2022, 11(10): 3482-3492.
  2. Weber A M, Kaiser J, Ziegler T, et al. A blue light receptor that mediates RNA binding and translational regulation[J]. Nature chemical biology, 2019, 15(11): 1085-1092.
  3. Dietler J, Gelfert R, Kaiser J, et al. Signal transduction in light-oxygen-voltage receptors lacking the active-site glutamine[J]. Nature Communications, 2022, 13(1): 2618.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 14
    Illegal SpeI site found at 103
    Illegal PstI site found at 348
    Illegal PstI site found at 1017
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 103
    Illegal PstI site found at 348
    Illegal PstI site found at 1017
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 61
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 14
    Illegal SpeI site found at 103
    Illegal PstI site found at 348
    Illegal PstI site found at 1017
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 14
    Illegal SpeI site found at 103
    Illegal PstI site found at 348
    Illegal PstI site found at 1017
    Illegal NgoMIV site found at 1891
    Illegal NgoMIV site found at 1963
    Illegal NgoMIV site found at 2053
    Illegal NgoMIV site found at 2071
    Illegal NgoMIV site found at 2583
    Illegal NgoMIV site found at 2876
    Illegal NgoMIV site found at 2970
    Illegal AgeI site found at 393
    Illegal AgeI site found at 678
    Illegal AgeI site found at 888
    Illegal AgeI site found at 999
    Illegal AgeI site found at 1605
    Illegal AgeI site found at 2751
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2640
    Illegal BsaI.rc site found at 1504
    Illegal BsaI.rc site found at 4110


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Categories
Parameters
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