Composite

Part:BBa_K4613307

Designed by: Qinfei Zhou   Group: iGEM23_NAU-CHINA   (2023-10-10)


Plac-RBS-yf1-RBS-fixJ-Pfixk2-RBS-GFP(YF1-GFP)

During the optimization of BBa_K4192130, mCherry, employed as the reporter gene, did not function effectively, greatly affecting our experiments. To better characterize the efficiency of this regulatory system, we replaced mCherry with GFP. The replacement of the reporter gene did not entail any changes to the regulatory system. After substitution, the system operated successfully.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 589
    Illegal NgoMIV site found at 661
    Illegal NgoMIV site found at 751
    Illegal NgoMIV site found at 769
    Illegal NgoMIV site found at 1281
    Illegal NgoMIV site found at 1574
    Illegal NgoMIV site found at 1668
    Illegal AgeI site found at 303
    Illegal AgeI site found at 1449
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1338
    Illegal BsaI.rc site found at 202
    Illegal BsaI.rc site found at 2808


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Categories
Parameters
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