Composite

Part:BBa_K4604023:Design

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-10-07)


piG_21 (tetR_bluB_riboK12_mazF_AmpProm_mazE)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 710
    Illegal BamHI site found at 2756
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1603
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2238
    Illegal BsaI site found at 2484
    Illegal BsaI site found at 3045


Design Notes

In order to protect our system from high toxin concentrations it is designed to constitutively produce an antitoxin (MazE) to neutralize the toxin (MazF) during the initial stage of AdoCbl production. Once produced, AdoCbl inhibits the toxin expression through binding to the negatively regulating riboswitch, leading to cell survival. In the absence of sufficient AdoCbl production, toxin levels rise due to an inactive riboswitch structure, inducing cell death.