Composite

Part:BBa_K4601223:Design

Designed by: Doriane Blaise   Group: iGEM23_Evry-Paris-Saclay   (2023-09-23)


sfGFP-LVAtag expression cassette under the control of the pOmpR promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 133
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

PCR amplifications and Golden Gate assembly

References