Composite

Part:BBa_K4601209:Design

Designed by: Doriane Blaise   Group: iGEM23_Evry-Paris-Saclay   (2023-09-23)


NpHR-P240T-F250Y-NpHTRII-EnvZ expression cassette in the Evolution.T7 mutational region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2274
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1301


Design Notes

Source

DNA synthesis, PCR amplifications and Golden Gate assembly

References