Part:BBa_K4601004

NpHR P240T F250Y

This part is the NpHR P240T F250Y mutant halorhodopsin.

Usage and Biology

NpHR is a halorhodopsin identified in the archaea Natronomonas pharaonis (Uniprot P15647). Its a mutant P240T, F250Y is able to interact with NpHtrII, while the wild type protein (BBa_K4601003) is not [1].

Halorhodopsins are chloride inward pumps activated by light [2].

For the characterisation of this part an expression cassette was assembled and the results are presented on its page in Parts Registry: BBa_K4601204.

References

[1] Sudo Y, Yamabi M, Kato S, Hasegawa C, Iwamoto M, Shimono K, Kamo N. Importance of specific hydrogen bonds of archaeal rhodopsins for the binding to the transducer protein. Journal of Molecular Biology (2006) 357: 1274–1282.

[2] Engelhard C, Chizhov I, Siebert F, Engelhard M. Microbial halorhodopsins: light-driven chloride pumps. Chemical Reviews (2018) 118: 10629–10645.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]