DNA

Part:BBa_K4600006

Designed by: Kavirang Biswas   Group: iGEM23_Warwick   (2023-10-11)


pMonix001

Contains a CpxR recognition site (CpxP), TetR sequence and a multidrug efflux pump (AcrB). This is Team BioMonix's construct aimed at allowing cells that produce perillyl alcohol to self regulate expression of enzymes that produce perillyl alcohol in order to tackle the toxicity issue of production.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 937
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 937
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2830
    Illegal XhoI site found at 773
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 937
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 937
    Illegal NgoMIV site found at 859
    Illegal NgoMIV site found at 1831
    Illegal NgoMIV site found at 1864
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None