DNA
Part:BBa_K4597001:Design
Designed by: Dylan Brettingham Group: iGEM23_Guelph (2023-09-16)
E.coli GIF - optomized
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The main design consideration was the optimization of the codons in the gene sequence to increase expression in the E. coli chassis we are using.
Source
This part comes from the human genomic sequence but has had some bases changed to optimize the codons for expression in E. coli. IDT's codon optimization tool was used for this.