Coding

Part:BBa_K4596004:Design

Designed by: Junteng Sun   Group: iGEM23_OUC-Haide   (2023-08-21)


NCD dependent formaldehyde dehydrogenase ->FalDH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 837
    Illegal AgeI site found at 325
    Illegal AgeI site found at 520
    Illegal AgeI site found at 1006
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The codons were optimized to enhance their expression in Escherichia coli.


Source

The gene sequence was found from genbank, and then modified according to the literature

References