DNA

Part:BBa_K4593005:Design

Designed by: Huyang Zhang   Group: iGEM23_BNDS-China   (2023-10-04)

PA#2/8


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added biotin at the 3’ end to test the affinity of the aptamer


Source

In 2016, Regina Stoltenburg and her team discovered PA#2/8 is an aptamer binding the S.aureus protein A.

References

[1] Stoltenburg, R., Krafčiková, P., Víglaský, V., & Strehlitz, B. (2016). G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONA. Scientific Reports, 6(1). https://doi.org/10.1038/srep33812