Part:BBa_K4592001
Microcystis-optimized eGFP CDS
This eGFP gene sequence has been optimized for expression and transformation in Microcystis aeruginosa. The nucleotide sequence has been passed through the Chameleon program developed by our team, which removed palindromic motifs that were underrepresented in the UTEX 2385 M. aeruginosa genome through synonymous codon-optimization, i.e. without altering the amino acid sequence. Therefore, the sequence has been codon-optimized for UTEX 2385 M. aeruginosa and has had sites potentially targeted by the strain's restriction-modification system removed without altering protein function. The removal of restriction sites is proposed to improve transformation efficiency in M. aeruginosa by preventing the sequence from being targeted by the organism's restriction enzymes; for this reason and the translation efficiency improvement with codon-optimization, this part is suggested as an improved fluorescent reporter for use in M. aeruginosa.
Usage and Biology
We were unable to assemble our plasmids and express them in UTEX 2385 M. aeruginosa before the Parts Pages Freeze and have therefore not been able to gather usage information for this part, however we plan to express the part in UTEX 2385 M. aeruginosa using the CaMV35S core promoter (BBa_K4592000) via plasmids containing our composite part (BBa_K4592002). This page will be updated in the future with this information.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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