Part:BBa_K4591010

Pm-MstX-BslA-tetR-Pxyl-cre-T500

Usage and Biology

In order to improve the degradation efficiency of engineered bacteria, we designed the gene circuit of attached module. The expression of genes related to this route can promote the formation of a biofilm on the surface of Bacillus subtilis, greatly improve the adhesion ability of engineered bacteria, reduce the mobility of engineered bacteria, and improve the degradation efficiency of PET. This module includes the Xylsmut-P_m biosensor, the membrane integrin MstX for membrane protein expression in Bacillus subtilis, and the hydrophobic protein BslA.

Basic components of the circuit

P_m-xylsmut: Xylsmut is a biosensor that recognizes TPA, a degradation product of PET, and activates the Pm promoter, which initiates the expression of downstream genes.

MstX: MstX increase the phosphorylation of Spo0A to express proteins and polysaccharides required for biofilm formation and prevent flagellar rotation. Promote the formation of engineered bacteria biofilm.

BslA: BslA has the ability to form a hydrophobic layer on the surface of Bacillus subtilis.

tetR:TetR is a secretion protein that binds to the upstream teto of the reporter module, inhibiting the expression of the fluorescent sfGFP gene.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2294