Coding

Part:BBa_K4585005

Designed by: Zijun Zhang   Group: iGEM23_CSU-CHINA   (2023-09-24)

Degron

Degron protein degradation sequence. Degron is a short peptide with a length of 41 amino acids that binds to E3 ubiquitin ligase through a disaster-to-order conversion, causing the substrate to be ubiquitinated and subsequently degraded.

Diagrams


Fig 1. The model diagram of Degron


Fig 2. The agarose gel electrophoresis maps of Degron fragment PCR products


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 92
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 92
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 92
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 92
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None