Coding
Part:BBa_K4585002:Design
Designed by: Siyao Ma Group: iGEM23_CSU-CHINA (2023-09-22)
VP64
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 138
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 138
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 138
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
GAL4 combining domain is universal among eukaryotic cells, but the activation factor needs to be chosen differently according to the chassis creature.
Source
VP64 is a transcription activation factor, originates from Vibrio parahaemolyticus. It can realize the activation of downstream UAS transcription. It is usually used together with GAL4 combining domain, which can recognize and combine with UAS, to locate UAS.