Composite

Part:BBa_K4577007

Designed by: Ma Bourgeois   Group: iGEM23_Ionis-Paris   (2023-10-06)


Hao gene with the promotor, terminator and the efficient RBS (g10-L) from bacteriophage T7

This composite contains the Hao gene flanked by the T7 promoter and terminator, which are derived from the T7 bactriophage. The main part is the Hao gene, which codes for the Hao protein. Its main function is to oxidise NH2OH to NO2-. [1]

The T7 promoter is a strong promoter. [2]

A ribosome binding site RBS (g10-L from the T7 bacteriophage) is also added between the promoter and the gene. [3]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1262
    Illegal XhoI site found at 1433
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 92
    Illegal BsaI site found at 322
    Illegal BsaI.rc site found at 191
    Illegal BsaI.rc site found at 758
    Illegal SapI site found at 1399


[edit]
Categories
Parameters
None