Part:BBa_K4577005

AmoA

The AmoA (Ammonia monooxygenase A) gene encodes the AMO enzyme, which is derived from Aeromonas hydrophila bacteria. The role of this enzyme is to carry out an ammynoxidation of NH3 to hydroxylamine NH2-OH. The molecule produced is used by the Hao enzyme. [1] Together with the hao gene, the AmoA gene functions as one of the main genes responsible for the process of ammonia oxidation in bacteria. This reaction is crucial for the conversion of ammonia to nitrite. Technical abbreviations are explained when first used.[1] In addition, this chain reaction has been adapted to break down the compound 17α-ethinylestradiol, also known as EE2.[2]

However, the AMO enzyme can also oxidise a number of other organic amines.

The codons have been optimised for the Sinorhizobium meliloti Sm1021 strain.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 151
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 3