DNA

Part:BBa_K4577004

Designed by: Maé Bourgeois   Group: iGEM23_Ionis-Paris   (2023-10-03)


Hao

This section details the functional role of the hao (hydroxylamine oxidoreductase) gene in encoding the Hao enzyme, which is derived from the Aeromonas hydrophila bacteria. Together with the amoA gene, the hao gene functions as one of the primary genes responsible for the process of ammonia oxidation in bacteria. The Hao enzyme is closely linked with the AMO enzyme, as it is capable of catalysing the oxidation of NH2OH (produced by AMO) into NO2-.[1]

Together with the amoA gene, the hao gene functions as one of the primary genes responsible for the process of ammonia oxidation in bacteria. This reaction is crucial in the transformation of ammonia into nitrite. Technical abbreviations will be explained upon their first usage. Additionally, this chain reaction has been adapted for the degradation of the compound 17α-ethinylestradiol, also known as EE2.[1]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1213
    Illegal XhoI site found at 1384
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 43
    Illegal BsaI site found at 273
    Illegal BsaI.rc site found at 142
    Illegal BsaI.rc site found at 709
    Illegal SapI site found at 1350


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Categories
Parameters
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