Composite
Mut

Part:BBa_K4575013:Design

Designed by: Sarah Hutzl, Sumohit Sharma   Group: iGEM23_Stuttgart   (2023-10-06)


Expression cassette for Mutanase expression in Pichia pastoris


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2440
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1224
    Illegal AgeI site found at 2161
    Illegal AgeI site found at 2594
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for Pichia pastoris. The introns and the signal peptide from the Mutanase gene were removed during the design process.


Source

Synthesized by IDT. Sequences from various genomic or synthetic backgrounds and sources (see basic part pages).

References