Coding
hIGHp

Part:BBa_K4574050

Designed by: Igor Koop   Group: iGEM23_Munich   (2023-10-06)


IGHV1-46 promoter

VH1-family human IGH promoter from human IGHV1-46 gene. It contains two cis-acting elements in addition to the TATA box: the octamer motif that regulates tissue-specific expression of IGH genes and the heptamer motif essential for full promoter activity. These elements are binding regions for octamer (Oct) transcription factors 1 and 2.

Source

The following basic part has a genomic origin[1].

Organism Homo sapiens (human)
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Gene IGH: immunoglobulin heavy locus (NCBI 3492)
Location 14q32.33, 106511598←106511752

It was first identified and sequenced by Haino et al.[2].

Usage and Biology

This basic part has been used as a component of several homology templates in RAMOS B-cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 100
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

  1. ↑ Gene [Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; 2004 – [cited 2023 Oct 01]. Available from: https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=3492
  2. ↑ Haino, M., Hayashida, H., Miyata, T., Shin, E. K., Matsuda, F., Nagaoka, H., Matsumura, R., Taka-ishi, S., Fukita, Y., & Fujikura, J. (1994). Comparison and evolution of human immunoglobulin VH segments located in the 3’ 0.8-megabase region. Evidence for unidirectional transfer of segmental gene sequences. Journal of Biological Chemistry, 269(4), 2619–2626. https://doi.org/10.1016/S0021-9258(17)41989-2


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Parameters
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