RNA
gRNA-hIGK1
Part:BBa_K4574036
Designed by: Alessio D. Nahmad Group: iGEM23_Munich (2023-10-06)
RNA guide hIGK-1
CRISPR guide RNA (gRNA), designed to target the human IGK locus, precisely the light chain kappa constant region (Lκ). When used with the CRISPR/Cas9 system, this guide sequence, 5'-TGGTGCAGCCACAGTTC^CTG(AGG)-3', ensures precise double-stranded break 3 bp upstream of the PAM recognition sequence (indicated in parentheses).
Source
The following basic part was designed to target genetic locus[1].
Organism | Homo sapiens (human) |
Taxonomy | Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo |
Gene | IGK: immunoglobulin kappa locus (NCBI 50802) |
Location | 2p11.2, 88857667→88857689 |
It was first identified and used by Nahmad et al.[2].
Usage and Biology
This basic part has been used as a component of a CRISPR knock-out system in RAMOS B-cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Gene [Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; 2004 – [cited 2023 Oct 01]. Available from: https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=50802
- ↑ Barzel, A., Nahmad, A. D., Akriv, T., Fried, M., Dotan, I., & Nataf, D. (2020). Genetic engneering of B cell receptors and uses thereof in antigen-induced antibody secretion (World Intellectual Property Organization Patent WO/2020/053865). https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2020053865
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Categories
Parameters
None |