Part:BBa_K4574029

XhoI restriction site

XhoI recognition site, a six-base restriction enzyme site with the sequence 5'-C^TCGAG-3', originating from Xanthomonas holcicola. It is cleaved after C-1 by a type IIP restriction endonuclease leaving 5' sticky ends.

Source

Common restriction site in molecular biology.

Usage and Biology

This basic part has been used as a component of several antibody constructs in mammalian cell culture (HEK293T, Exi293, RAMOS).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]