Part:BBa_K4574026
T2A
A 2A-like ribosomal skipping sequence derived from the Thosea asigna virus. When translated, this specialized peptide-encoding sequence causes ribosomes to 'skip' the formation of a terminal Gly-Pro peptide bond, leading to the equimolar co-expression of two separate polypeptides from a single mRNA.
Source
The following basic part has a genomic origin[1].
| Organism | Thosea asigna virus |
| Taxonomy | Viruses; Riboviria; Orthornavirae; Permutotetraviridae; Alphapermutotetravirus |
| Gene | RdRp: RNA-dependent RNA polymerase (NCBI 41332125) |
| Location | 3734→3787 |
Its use in polycistronic vectors for recombinant proteins was described by Liu et al.[2]
The provided sequence was derived through back-translation from the resulting peptide. This back-translation took into account the codon usage bias for expression in mammalian cells (see "Usage and Biology" section). Additionally, the sequence was designed to comply with the BioBrick assembly standards.
Usage and Biology
This basic part has been used as a component of several antibody constructs as well as CRISPR homology-directed repair templates in mammalian cell culture (HEK293T, Exi293, RAMOS).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Gene [Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; 2004 – [cited 2023 Oct 01]. Available from: https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=41332125
- ↑ Liu, Z., Chen, O., Wall, J. B. J., Zheng, M., Zhou, Y., Wang, L., Ruth Vaseghi, H., Qian, L., & Liu, J. (2017). Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector. Scientific Reports, 7(1), Article 1. https://doi.org/10.1038/s41598-017-02460-2
| None |