Part:BBa_K4574020

11aa-APG-Linker

APG-repeat based short linker designed for seamless fusion of immunoglobulins to single-chain variable fragments (scFv). It possesses the proper length and flexibility to optimise the functionality of the connected domains.

Source

The following basic part was developed for scFv fusion to an antibody by Hultqvist et al.^[1].

The provided sequence was derived through back-translation from the resulting peptide. This back-translation took into account the codon usage bias for expression in mammalian cells (see "Usage and Biology" section). Additionally, the sequence was designed to comply with the BioBrick assembly standards.

Usage and Biology

This basic part has been used as a component of several antibody constructs in mammalian cell culture (HEK293T, Exi293, RAMOS).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

↑ Rofo, F., Meier, S. R., Metzendorf, N. G., Morrison, J. I., Petrovic, A., Syvänen, S., Sehlin, D., & Hultqvist, G. (2022). A Brain-Targeting Bispecific-Multivalent Antibody Clears Soluble Amyloid-Beta Aggregates in Alzheimer’s Disease Mice. Neurotherapeutics, 19(5), 1588–1602. https://doi.org/10.1007/s13311-022-01283-y

[1] Rofo, F., Meier, S. R., Metzendorf, N. G., Morrison, J. I., Petrovic, A., Syvänen, S., Sehlin, D., & Hultqvist, G. (2022). A Brain-Targeting Bispecific-Multivalent Antibody Clears Soluble Amyloid-Beta Aggregates in Alzheimer’s Disease Mice. Neurotherapeutics, 19(5), 1588–1602. https://doi.org/10.1007/s13311-022-01283-y

[1]