Part:BBa_K4574018
IGLC2
Human immunoglobulin light chain constant lambda (Cλ) region derived from the IGLC2 gene. This sequence represents the constant domain of the lambda light chain, integral to the structure and function of antibodies.
Source
The following basic part has a genomic origin[1].
| Organism | Homo sapiens (human) |
| Taxonomy | Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo |
| Gene | IGLC2: immunoglobulin lambda constant 2 (NCBI 3538) |
| Location | 22q11.22, 22900975→22901292 |
It was first identified and sequenced by Milstein et al.[2]
The provided sequence was derived through back-translation from the resulting peptide. This back-translation took into account the codon usage bias for expression in mammalian cells (see "Usage and Biology" section). Additionally, the sequence was designed to comply with the BioBrick assembly standards.
Usage and Biology
This basic part has been used as a component of several antibody constructs in mammalian cell culture (HEK293T, Exi293, RAMOS).
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 55
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 55
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 55
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 55
Illegal NgoMIV site found at 151
Illegal AgeI site found at 245 - 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Gene [Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; 2004 – [cited 2023 Oct 01]. Available from: https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=3538
- ↑ Milstein, C., Clegg, J. B., & Jarvis, J. M. (1968). Immunoglobulin λ-chains. The complete amino acid sequence of a Bence-Jones protein. Biochemical Journal, 110(4), 631–652. https://doi.org/10.1042/bj1100631
| None |