Part:BBa_K4574008
Anti-hTfR IGVL
Variable light chain locus of the humanized hu128.1 monoclonal IgG1 antibody, which specifically targets human transferrin receptor (hTfR). This component is integral to a therapeutic antibody capable of acting as a blood-brain-barrier shuttle.
Source
The following basic part was developed for the therapeutic monoclonal antibody shuttle binding to hTfR by Dengl et al.[1].
The provided sequence was derived through back-translation from the resulting peptide. This back-translation took into account the codon usage bias for expression in mammalian cells (see "Usage and Biology" section). Additionally, the sequence was designed to comply with the BioBrick assembly standards.
Usage and Biology
This basic part has been used as a component of several antibody constructs in mammalian cell culture (HEK293T, Exi293, RAMOS).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Dengl, S., Georges, G., Goepfert, U., Niewoehner, J., & Schlothauer, T. (2016). Anti-transferrin receptor antibodies with tailored affinity (World Intellectual Property Organization Patent WO2016207240A1). https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2016207240
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