Part:BBa_K4574002

IGHV1-51 leader

Human heavy chain (HC) leader sequence from the GHV1-51 gene — an N-terminal signal peptide associated with the heavy chain of antibodies. This leader sequence facilitates the translocation of the heavy chain into the endoplasmic reticulum, ensuring its proper folding, assembly, and secretion in recombinant expression systems.

Source

The following basic part has a genomic origin^[1].

Organism	Homo sapiens (human)
Taxonomy	Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Gene	IGH: immunoglobulin heavy locus (NCBI 3492)
Location	14q32.33, 106579038←106579177

It was first identified and sequenced by Haino et al.^[2]

The provided sequence was derived through back-translation from the resulting peptide. This back-translation took into account the codon usage bias for expression in mammalian cells (see "Usage and Biology" section). Additionally, the sequence was designed to comply with the BioBrick assembly standards.

Usage and Biology

This basic part has been used as a component of several antibody constructs in mammalian cell culture (HEK293T, Exi293, RAMOS).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 40
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 40
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 40
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 40
1000
COMPATIBLE WITH RFC[1000]

References

↑ Gene [Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; 2004 – [cited 2023 Oct 01]. Available from: https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=3492
↑ Haino, M., Hayashida, H., Miyata, T., Shin, E. K., Matsuda, F., Nagaoka, H., Matsumura, R., Taka-ishi, S., Fukita, Y., & Fujikura, J. (1994). Comparison and evolution of human immunoglobulin VH segments located in the 3’ 0.8-megabase region. Evidence for unidirectional transfer of segmental gene sequences. Journal of Biological Chemistry, 269(4), 2619–2626. https://doi.org/10.1016/S0021-9258(17)41989-2

[source-1] Gene [Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; 2004 – [cited 2023 Oct 01]. Available from: https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=3492

[2] Haino, M., Hayashida, H., Miyata, T., Shin, E. K., Matsuda, F., Nagaoka, H., Matsumura, R., Taka-ishi, S., Fukita, Y., & Fujikura, J. (1994). Comparison and evolution of human immunoglobulin VH segments located in the 3’ 0.8-megabase region. Evidence for unidirectional transfer of segmental gene sequences. Journal of Biological Chemistry, 269(4), 2619–2626. https://doi.org/10.1016/S0021-9258(17)41989-2

[1]

[2]