Part:BBa_K4574001
IGLV1-40 leader
Human variable lambda (Vλ) leader sequence from the IGLV1-40 gene — an N-terminal signal peptide associated with the lambda light chain of antibodies. This leader sequence facilitates the translocation of the lambda light chain into the endoplasmic reticulum, ensuring its proper folding, assembly, and secretion in recombinant expression systems.
Source
The following basic part has a genomic origin[1].
| Organism | Homo sapiens (human) |
| Taxonomy | Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo |
| Gene | IGL: immunoglobulin lambda locus (NCBI 3535) |
| Location | 22q11.22, 22409817→22409994 |
It was first identified and sequenced by Williams et al.[2]
The provided sequence was derived through back-translation from the resulting peptide. This back-translation took into account the codon usage bias for expression in mammalian cells (see "Usage and Biology" section). Additionally, the sequence was designed to comply with the BioBrick assembly standards.
Usage and Biology
This basic part has been used as a component of several antibody constructs in mammalian cell culture (HEK293T, Exi293, RAMOS).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Gene [Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; 2004 – [cited 2023 Oct 01]. Available from: https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=3535
- ↑ Williams, S. C., Frippiat, J.-P., Tomlinson, I. M., Ignatovich, O., Lefranc, M.-P., & Winter, G. (1996). Sequence and Evolution of the Human Germline Vλ Repertoire. Journal of Molecular Biology, 264(2), 220–232. https://doi.org/10.1006/jmbi.1996.0636
| None |