Plasmid_Backbone
pcDNA3.4

Part:BBa_K4574000:Design

Designed by: Invitrogen   Group: iGEM23_Munich   (2023-10-05)


pcDNA3.4-TOPO vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5993
    Illegal SpeI site found at 5334
    Illegal PstI site found at 1965
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 5334
    Illegal PstI site found at 1965
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 915
    Illegal BglII site found at 5097
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5993
    Illegal SpeI site found at 5334
    Illegal PstI site found at 1965
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5993
    Illegal SpeI site found at 5334
    Illegal PstI site found at 1965
    Illegal NgoMIV site found at 558
    Illegal NgoMIV site found at 1075
    Illegal NgoMIV site found at 2416
    Illegal NgoMIV site found at 2701
    Illegal AgeI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 798
    Illegal BsaI.rc site found at 4229
    Illegal SapI site found at 3146
    Illegal SapI.rc site found at 2265
    Illegal SapI.rc site found at 2475


Design Notes

See main page.

Source

See main page.

References

See main page.