Plasmid_Backbone
pcDNA3.4
Part:BBa_K4574000:Design
Designed by: Invitrogen Group: iGEM23_Munich (2023-10-05)
pcDNA3.4-TOPO vector
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 5993
Illegal SpeI site found at 5334
Illegal PstI site found at 1965 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SpeI site found at 5334
Illegal PstI site found at 1965 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BglII site found at 915
Illegal BglII site found at 5097 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 5993
Illegal SpeI site found at 5334
Illegal PstI site found at 1965 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 5993
Illegal SpeI site found at 5334
Illegal PstI site found at 1965
Illegal NgoMIV site found at 558
Illegal NgoMIV site found at 1075
Illegal NgoMIV site found at 2416
Illegal NgoMIV site found at 2701
Illegal AgeI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 798
Illegal BsaI.rc site found at 4229
Illegal SapI site found at 3146
Illegal SapI.rc site found at 2265
Illegal SapI.rc site found at 2475
Design Notes
See main page.
Source
See main page.
References
See main page.