Plasmid_Backbone

Part:BBa_K4516006:Design

Designed by: Kong Yangyang   Group: iGEM22_Jiangsu_United   (2022-07-19)


pcDNA3.1-backbone


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 312
    Illegal PstI site found at 1647
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 292
    Illegal PstI site found at 1647
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 12
    Illegal XhoI site found at 318
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 312
    Illegal PstI site found at 1647
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 312
    Illegal PstI site found at 1647
    Illegal NgoMIV site found at 757
    Illegal NgoMIV site found at 2098
    Illegal NgoMIV site found at 2381
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 279
    Illegal BsaI.rc site found at 3906
    Illegal SapI site found at 2826
    Illegal SapI.rc site found at 1947
    Illegal SapI.rc site found at 2157


Design Notes

No


Source

No

References