Plasmid

Part:BBa_K4514052:Design

Designed by: Daria Perminova   Group: iGEM22_MiamiU   (2022-10-12)


CRISPR-Cas13a expression vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 207
    Illegal XbaI site found at 236
    Illegal XbaI site found at 3088
    Illegal XbaI site found at 3172
    Illegal SpeI site found at 4045
    Illegal SpeI site found at 7135
    Illegal PstI site found at 6289
    Illegal PstI site found at 7884
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 207
    Illegal SpeI site found at 4045
    Illegal SpeI site found at 7135
    Illegal PstI site found at 6289
    Illegal PstI site found at 7884
    Illegal NotI site found at 6307
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 207
    Illegal BglII site found at 22
    Illegal BamHI site found at 1275
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 207
    Illegal XbaI site found at 236
    Illegal XbaI site found at 3088
    Illegal XbaI site found at 3172
    Illegal SpeI site found at 4045
    Illegal SpeI site found at 7135
    Illegal PstI site found at 6289
    Illegal PstI site found at 7884
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 207
    Illegal XbaI site found at 236
    Illegal XbaI site found at 3088
    Illegal XbaI site found at 3172
    Illegal SpeI site found at 4045
    Illegal SpeI site found at 7135
    Illegal PstI site found at 6289
    Illegal PstI site found at 7884
    Illegal NgoMIV site found at 7433
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5980
    Illegal BsaI.rc site found at 5968
    Illegal SapI site found at 7373
    Illegal SapI site found at 7583


Design Notes

N/A


Source

From (Kiga et al., 2020). Ordered off AddGene

References