Coding
aphA

Part:BBa_K4509369:Design

Designed by: Rhea Harry William, MARIE JYOTSNA JAWAHAR, Vibha Murali   Group: iGEM22_REC-CHENNAI   (2022-09-24)


Acid phosphatase (aphA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 433


Design Notes

The sequence naturally contained restriction enzyme recognition sites which were incompatible with certain assembly standards. To over come this, the sequence was codon optimized to be compatible with all assembly standards.


References

1) Hofsten, B. V. (1961). Acid phosphatase and the growth of Escherichia coli. Biochimica et Biophysica Acta, 48(1), 171-181.

2) KOstanin, E.H.Harms, P.E.Stevis, RKuciel, M.M.Zhou, R.L.Van Etten.15 November 1992.Overexpression, site-directed mutagenesis, and mechanism of Escherichia coli acid phosphatase. Journal of Biological Chemistry.

3)M C Thaller , S Schippa, A Bonci, S Cresti, G M Rossolini. 1997 Jan. Identification of the gene (aphA) encoding the class B acid phosphatase/phosphotransferase of Escherichia coli MG1655 and characterization of its product.146(2):191-8.

4)Schmidt, G., Bartsch, G., Laumont, M. C., Herman, T., & Liss, M. (1963). Acid phosphatase of bakers' yeast: an enzyme of the external cell surface. Biochemistry, 2(1), 126-131.