Coding

Part:BBa_K4502001

Designed by: liu jinrong   Group: iGEM22_Thinker-Shenzhen   (2022-10-09)


Added by Thinker-Shenzhen

After obtaining the modified chitinase again, we repeated the test in the first round using the control and experimental groups and compared the data from the two experiments.

The binding capacity of the modified chitinase.

Figure 1

Modified chitinase activity.

Figure 2
Figure 3

Inhibition of living fungi by modified chitinases.

Figure 4

We can see that after using the modification the titinase has improved dramatically in terms of binding capacity and activity, which meets the needs of our project.


chitinase

The N-terminus uses the binding domain of chitinase derived from Bacillus cereus In the middle is the degradation domain of chitinase derived from Streptomyces alfalfa The C-terminus uses the chitinase binding domain of Bacillus Thuringiensis

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 538
    Illegal NotI site found at 86
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 596
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 456
  • 1000
    COMPATIBLE WITH RFC[1000]


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