Device

Part:BBa_K4501005:Design

Designed by: Pau Marín Escudero   Group: iGEM22_Barcelona_UB   (2022-09-08)


MSCV 5' module


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 268
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 780


Design Notes

The sequence was domesticated to eliminate any BbsI, BsmBI and AarI recognition sites.


Source

EF.CMV.RFP was a gift from Linzhao Cheng (Addgene plasmid # 17619 ; http://n2t.net/addgene:17619 ; RRID:Addgene_17619) Lentiviral vectors with two independent internal promoters transfer high-level expression of multiple transgenes to human hematopoietic stem-progenitor cells. Yu X, Zhan X, D'Costa J, Tanavde VM, Ye Z, Peng T, Malehorn MT, Yang X, Civin CI, Cheng L. Mol Ther. 2003 Jun . 7(6):827-38. 10.1016/S1525-0016(03)00104-7 PubMed 12788657

References